A method for the detection and enrichment of endogenous cereblon substrates.
Lloyd HC, Li Y, Payne NC, Zhao Z, Xu W, Kroupova A, Zollman D, Long T, Kabir F, Chen M, Freeman R, Feng EY, Xi SY, Hsu YC, Ciulli A, Mazitschek R, Woo CM
Cell Chem Biol. 2025 Aug 5:S2451-9456(25)00225-9. doi: 10.1016/j.chembiol.2025.07.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236737 | pET28a-TBD | Protein overexpression of Thalidomide Binding domain of hCRBN |
| 236738 | pET28a-cerebody | Protein overexpression of cerebody |
| 236739 | pET28a-His6-UROD | Protein overexpression of human uroporphyrinogen decarboxylase |
| 236740 | pET28a-His6-UROD-N367A | Protein overexpression of human uroporphyrinogen decarboxylase N367A |
| 236741 | pET28a-His6-GFP-UROD | Protein overexpression of eGFP-human uroporphyrinogen decarboxylase |
| 236742 | pET28a-His6-GFP-UROD-N367A | Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A |