Protocol for CRISPR-based manipulation and visualization of endogenous alpha-synuclein in cultured mouse hippocampal neurons.
Parra-Rivas LA, Sharma R, Rust TE, Bazick HO, Carlson-Stevermer J, Zylka MJ, Ogawa Y, Roy S
STAR Protoc. 2025 Jul 21;6(3):103945. doi: 10.1016/j.xpro.2025.103945.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239403 | pLP857_α-syn:oScarlet KI donor | AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn |
| 239417 | pLP16_Lenti Scramble Control | Negative control Lentiviral plasmid for SpCas9-based CRISPR KO |
| 239419 | pLP110_AAV Scramble Control | Negative control AAV vector for SaCas9-based CRISPR KO |