CRISPR GENome and epigenome engineering improves loss-of-function genetic-screening approaches.
Stadager J, Bernardini C, Hartmann L, May H, Wiepcke J, Kuban M, Najafova Z, Johnsen SA, Legewie S, Traube FR, Jude J, Rathert P
Cell Rep Methods. 2025 Jun 16;5(6):101078. doi: 10.1016/j.crmeth.2025.101078. Epub 2025 Jun 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239603 | EF1as-ZIM3-Cas9-P2A-GFP | Constitutive active CRISPRgenee construct |
| 239604 | TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti | Doxycycline inducible CRISPRgenee construct with a blasticidin resistance |
| 239605 | TRE3G-ZIM3-Cas9-P2A-GFP | Doxycycline inducible CRISPRgenee construct |
| 239608 | sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo | Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance |
| 239609 | sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo | Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance |
| 239610 | EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti | Constitutive active CRISPRgenee construct with a blasticidin resistance |