Adaptive evolution of CENP-T modulates centromere binding.
Dudka D, Nguyen AL, Boese KG, Marescal O, Akins RB, Black BE, Cheeseman IM, Lampson MA
Curr Biol. 2025 Mar 10;35(5):1012-1022.e5. doi: 10.1016/j.cub.2025.01.017. Epub 2025 Feb 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237426 | pGEMHE-MmCENPT-GFP | Expresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237427 | pGEMHE-MmCENPT-MsHFD-GFP | Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237428 | pGEMHE-MmCENPT-RpHFD-GFP | Expresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237429 | pGEMHE-MmCENPT-RnHFD-GFP | Expresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237430 | pGEMHE-MmCENPT-ASD2HFD-GFP | Expresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237431 | pGEMHE-MmCENPT-4NS-GFP | Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237432 | pGEMHE-MmCENPT-RnHFD-4NS-GFP | Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 237433 | pGEMHE-MmCENP-W | Expresses Mus musculus CENP-W; untagged; made for in vitro transcription |
| 237437 | pGEMHE-MmCENPT-McHFD-GFP | Expresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter) |
| 238064 | pGEMHE-RnCENP-W | Expresses rat CENP-W; untagged; made for in vitro transcription |
| 239798 | pALN130 | Express mEGFP tagged mouse CENPT |
| 239799 | pALN132 | Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain |
| 239800 | pALN148 | Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain and 4 non-synonymous substitutions |