Condensate interfacial forces reposition DNA loci and probe chromatin viscoelasticity.
Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, Brangwynne CP
Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227376 | NLS-GFP-iLID-Ferritin | Condensate module, light-inducible oligomerization 'core' |
| 227377 | FUS-mCh-sspB | Condensate module, light-inducible oligomerization IDR |
| 227378 | FUS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227379 | FUS-3YS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227380 | FUS-5YS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227381 | FUS-9YS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227382 | FUS-15YS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227383 | FUS-27YS-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227384 | HNRNPA1-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227385 | iLID-miRFP670-TRF1 | Adhesion module, creates 'seed' at telomere |
| 227386 | FUS-miRFP670-Coilin | Adhesion module, creates 'seed' at Cajal body |
| 227387 | BRD4dN-mCherry-SspB | Condensate module, light-inducible oligomerization IDR |
| 227388 | miRFP670-TRF1 | Label telomere, no adhesion |
| 227389 | dCas9-SunTag(10X)-2A-BFP | Adhesion module, dCas9-SunTag |
| 227390 | scFv-HaloTag-iLID | Adhesion module, scFv binds SunTag |
| 227391 | sgPPP1R2 | Adhesion module, guide RNA recognizes PPP1R2 |
| 227392 | sgTelo | Adhesion module, guide RNA recognizes telomeres |