Directed evolution of hyperactive integrases for site specific insertion of transgenes.
Hew BE, Gupta S, Sato R, Waller DF, Stoytchev I, Short JE, Sharek L, Tran CT, Badran AH, Owens JB
Nucleic Acids Res. 2024 Jul 2:gkae534. doi: 10.1093/nar/gkae534.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242869 | pCMV2 HuOpt Bxb1 | Mammalian expression of Bxb1 Integrase |
| 242870 | pCMV2 Bxb1-L7-3 | Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A425T) |
| 242871 | pCMV2 Bxb1-c2 | Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L) |
| 242872 | pCMV2 Bxb1-c10 | Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G) |
| 242873 | pCMV2 Bxb1-c15 | Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G) |
| 242874 | pCMV2 Bxb1-c22 | Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A369P, E434G) |
| 242875 | pCMV2 Bxb1-c26 | Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, A369P, 434G) |
| 242878 | pU6 pegRNA ROSA 4a Bxb1 attB38 | Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site |
| 242879 | pU6 pegRNA ROSA 4b Bxb1 attB38 | Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site |
| 242880 | pU6 pegRNA ROSA 4a PhiC31 attP41 | Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site |
| 242881 | pU6 pegRNA ROSA 4b PhiC31 attP41 | Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site |
| 242882 | pU6 pegRNA AAV 4a PhiC31 attP41 | Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site |
| 242883 | pU6 pegRNA AAV 3b PhiC31 attP41 | Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site |
| 242884 | pCMV Bxb1 attP dsRed Hygro | Selectable donor plasmid for insertion by Bxb1 integrase into pre-inserted attB landing pad site |
| 242885 | pCMV PhiC31 attB dsRed Hygro | Selectable donor plasmid for insertion by PhiC31 integrase into pre-inserted attP landing pad site |