Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs.
Li Y, Tan M, Akkari-Henic A, Zhang L, Kip M, Sun S, Sepers JJ, Xu N, Ariyurek Y, Kloet SL, Davis RP, Mikkers H, Gruber JJ, Snyder MP, Li X, Pang B
Nat Biomed Eng. 2024 May 22. doi: 10.1038/s41551-024-01204-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218930 | Lenti-dual-CRISPR-U6-H1(pBP43) | The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries. |
| 218931 | pUC19-dual-scafffold-2.0 (pBP49) | The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids. |
| 218932 | Dual-CRISPR-Cas9-U6-H1 (pBP48) | The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids. |