A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.
Victoria AJ, Selao TT, Moreno-Cabezuelo JA, Mills LA, Gale GAR, Lea-Smith DJ, McCormick AJ
Plant Physiol. 2024 May 7:kiae261. doi: 10.1093/plphys/kiae261.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203934 | pC0.420 | Up Flank. Neutral site. Up flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901. |
| 203935 | pC0.419 | Down Flank. Neutral site. Down flanking sequence of type II site-specific deoxyribonuclease AquI (FEK30_10065) in Synechococcus sp. PCC 11901. |
| 203936 | pC0.385 | Up Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901. |
| 203937 | pC0.378 | Down Flank. Neutral site. Down flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901. |
| 203938 | pC0.386 | Up Flank. Neutral site. Up flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901. |
| 203939 | pC0.387 | Down Flank. Neutral site. Down flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901. |
| 203940 | pC0.388 | Up Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901. |
| 203941 | pC0.389 | Down Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901. |
| 203942 | pC0.437 | Up Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901. |
| 203943 | pC0.438 | Down Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901. |
| 203944 | pC0.425 | Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR. |
| 203945 | pC0.426 | Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR. |
| 203946 | pC0.422 | Level 0 part. Rhamnose-inducible rhaS_PrhaBAD promoter. |
| 203947 | pC0.423 | Level 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter. |
| 203948 | pC0.391 | Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position |
| 203949 | pC0.393 | Level 0 part. Gentamicin resistance cassette in CDS2 position |
| 203950 | pCA0.421 | Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position |
| 203951 | pCA1.339 | Spectinomycin-based RSF1010 acceptor Level 1 |
| 203952 | pCAT.482 | Spectinomycin-based RSF1010 acceptor Level T |
| 203953 | pCA1.327 | Level 1 Position 1 pUC19 acceptor vector |
| 203954 | pC0.394 | Level 0 part. theophylline responsive riboswitch E* promoter. |
| 203955 | pC1.509 | Level 1 self-replicating vector containing a DAPG inducible FnCas12a |
| 205441 | pC1.530 | Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal. |