Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma.
Pulice JL, Meyerson M
bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221465 | pLX302_CdTag-HA_EmptyVector | Gateway backbone for C-terminal dTag cloning |
| 221466 | pLX304_CdTag-HA_EmptyVector | Gateway backbone for C-terminal dTag cloning |
| 221467 | pLX307_CdTag-HA_EmptyVector | Gateway backbone for C-terminal dTag cloning |
| 221468 | pLX311_CdTag-HA_EmptyVector | Gateway backbone for C-terminal dTag cloning |
| 221469 | pDONR223_GFP | Gateway cloning of GFP overexpression |
| 221470 | pDONR223_mCherry | Gateway cloning of mCherry overexpression |
| 221471 | pDONR223_NKX2-1_Long | Gateway cloning of NKX2-1 long isoform overexpression |
| 221472 | pDONR223_NKX2-1 | Gateway cloning of NKX2-1 short isoform overexpression |
| 221473 | pDONR223_NKX2-1_V205F | Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression |
| 221474 | pDONR223_NKX2-1_W208L | Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression |
| 221475 | pDONR223_NKX2-1_DelNTerm_160-371AA | Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression |
| 221476 | pDONR223_NKX2-1_DelCTerm_1-223AA | Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression |
| 221477 | pDONR223_NKX2-1_HD_160-223AA | Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression |
| 221478 | pDONR223_NKX2-4 | Gateway cloning of NKX2-4 overexpression |
| 221479 | pDONR223_NKX2-5 | Gateway cloning of NKX2-5 overexpression |
| 221480 | pLX-EF1a_Empty | Empty vector control for pLX307 overexpresion experiments |
| 221481 | pLX-EF1a_GFP | GFP lentiviral overexpression |
| 221482 | pLX-EF1a_mCherry | mCherry lentiviral overexpression |
| 221483 | pLX-EF1a_NKX2-1_Long | NKX2-1 long isoform lentiviral overexpression |
| 221484 | pLX-EF1a_NKX2-1 | NKX2-1 short isoform lentiviral overexpression |
| 221485 | pLX-EF1a_NKX2-1_V205F | NKX2-1 V205F DNA binding mutation lentiviral overexpression |
| 221486 | pLX-EF1a_NKX2-1_W208L | NKX2-1 W208L DNA binding mutation lentiviral overexpression |
| 221487 | pLX-EF1a_NKX2-1_DelNTerm_160-371AA | NKX2-1 N-terminal truncation mutation lentiviral overexpression |
| 221488 | pLX-EF1a_NKX2-1_DelCTerm_1-223AA | NKX2-1 C-terminal truncation mutation lentiviral overexpression |
| 221489 | pLX-EF1a_NKX2-1_HD_160-223AA | NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression |
| 221490 | pLX-EF1a_NKX2-4 | NKX2-4 lentiviral overexpression |
| 221491 | pLX-EF1a_NKX2-5 | NKX2-5 lentiviral overexpression |
| 221492 | pLX-EF1a_GFP_CdTag-HA | GFP lentiviral overexpression of C-terminal dTag fusion |
| 221493 | pLX-EF1a_NKX2-1_CdTag-HA | NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion |
| 221494 | pLX-hPGK_NKX2-1 | NKX2-1 short isoform lentiviral overexpression using hPGK promoter |
| 221495 | pLX-UBC_NKX2-1 | NKX2-1 short isoform lentiviral overexpression using UBC promoter |
| 221496 | pLX-SFFV_NKX2-1 | NKX2-1 short isoform lentiviral overexpression using SFFV promoter |
| 221497 | pLX-EF1a_NKX2-1_Short_V5-GFP | NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter |
| 221498 | pLX-hPGK_NKX2-1_Short_V5-GFP | NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter |
| 221499 | pLX-UBC_NKX2-1_Short_V5-GFP | NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter |
| 221500 | pLX-SFFV_NKX2-1_Short_V5-GFP | NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter |
| 221501 | pLenti_dCas9-KRAB-MeCP2_Blast | Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector |