Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform.
Kipfer ET, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T
Elife. 2023 Nov 21;12:RP89035. doi: 10.7554/eLife.89035.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211731 | pUC19_CoV-2_linker | Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions |
| 253119 | pUC19_CoV-2_frD1_XBB.1.5 S | For recombinant virus rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Omicron isolate XBB.1.5 Spike |
| 253129 | pUC19_CoV-2_frD2_ΔORF68 | For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf6 and orf8 |
| 253155 | pUC19_CoV-2_frD2_ΔrE/N1 Δ6 3* Δ7a/H1 WSN | Virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of env (residual AA), orf6, orf7a; stop codon at AA213 in orf3a;Influenza N1 (WSN) replaces env;H1 (WSN) replaces orf7a |
| 253170 | pUC19_CoV-2_ frD2_ΔE67 3* VSVG-P2A-N | For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein fused to N, P2A |