A novel interaction between RAD23A/B and Y-family DNA polymerases.
Ashton NW, Jaiswal N, Cestari Moreno N, Semenova IV, D'Orlando DA, Teatin Latancia M, McIntyre J, Woodgate R, Bezsonova I
J Mol Biol. 2023 Nov 5:168353. doi: 10.1016/j.jmb.2023.168353.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201438 | pCMV6-AN-DDK_WT RAD23A | Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells |
| 201439 | pCMV6-AN-DDK_WT RAD23B | Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells |
| 201440 | pCMV6-AN-HA_WT POLI | Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells |
| 201444 | pJM919_WT His POLI | Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag |
| 201445 | pJRM919_WT aa 1-419 His POLI | Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI |
| 201456 | pGEX-6P-1_WT RAD23A | Expresses human RAD23A with an N-terminal GST tag in E. coli |
| 201457 | pGEX-6P-1_WT RAD23B | Expresses human RAD23B with an N-terminal GST tag in E. coli |
| 201458 | pCMV6-AN-HA_WT RAD23A | Expresses human RAD23A with an N-terminal HA tag in mammalian cells |
| 201459 | pCMV6-AN-HA_WT RAD23B | Expresses human RAD23B with an N-terminal HA tag in mammalian cells |
| 201460 | pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain) | Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201463 | pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain) | Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201464 | pCMV6-AN-DDK_WT RAD23A_delta aa 230-285 (delta XPC-binding domain) | Expresses a mutant form of human RAD23A that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201465 | pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain) | Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201466 | pCMV6-AN-DDK_WT RAD23A_delta aa 111-136 | Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201467 | pCMV6-AN-DDK_WT RAD23A_delta aa 137-160 | Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201468 | pCMV6-AN-DDK_WT RAD23A_delta aa 202-230 | Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A. |
| 201469 | pCMV6-AN-DDK_WT RAD23A_delta aa 287-316 | Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201545 | pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain) | Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201546 | pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain) | Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201547 | pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain) | Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201548 | pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain) | Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201549 | pet28b_His_RAD23A-UBA1 | Expresses UBA1 of human RAD23A in E. coli |
| 201550 | pet28b-His_RAD23A-UBA2 | Expresses UBA2 of human RAD23A in E. coli |
| 201574 | pCMV6-AN-DDK_S172A_M173A RAD23A | Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201575 | pCMV6-AN-DDK_V195A RAD23A | Expresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201576 | pCMV6-AN-DDK_S172A_M173A_V195A RAD23A | Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201577 | pCMV6-AN-DDK_C344A RAD23A | Expresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201578 | pCMV6-AN-DDK_F354A RAD23A | Expresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201579 | pCMV6-AN-DDK_C344A_F354A RAD23A | Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A |
| 201580 | pCMV6-AN-DDK_V222A RAD23B | Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201581 | pCMV6-AN-DDK_F400A RAD23B | Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B |
| 201582 | pCMV6-AN-HA_aa 1-420 POLI | Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells |
| 201583 | pCMV6-AN-HA_aa 1-449 POLH | Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells |
| 201584 | pCMV6-AN-HA_WT POLK | Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells |
| 201585 | pCMV6-AN-HA_aa 1-542 POLK | Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells |
| 201586 | pCMV6-AN-HA_WT REV1 | Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells |
| 201587 | pCMV6-AN-HA_aa 321-843 REV1 | Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells |