Skip to main content
Addgene

Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.

Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J
Nat Methods. 2023 Dec;20(12):1900-1908. doi: 10.1038/s41592-023-02053-0. Epub 2023 Nov 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197056pcDNA3.1_GEM2-Halo-FRBExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells
197057pcDNA3.1_GEM4-Halo-FRBExpression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells
197058pcDNA3.1_GEM7-Halo-FRBExpression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells
197059pcDNA3.1_GEM22-Halo-FRBExpression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells
197060pcDNA3.1_FRB-GEM23-HaloExpression of nanocage I3-01 Halo-FRB-fusion in mammalian cells
197061pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
197062pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
197063pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
197064pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
197065pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)
197066pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-BlastDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP
197067pAAVS1_EF1a-MitoTrap-mCherry-IRES-PuroDonor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Antibodies from Article