FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways.

Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
224377	pX459_GNB1L_gRNA	Targets GNB1L for dTAG C terminal knock-in cell lines
224378	pUC19_GNB1L_dTAG	Generates GNB1L dTAG C terminal knock-in cell lines
224379	pX459_TELO2_gRNA	Targets TELO2 for dTAG N terminal knock-in cell lines
224380	pUC19_TELO2_dTAG	Generates TELO2 dTAG N terminal knock-in cell lines
224381	pLenti_CMV_Puro_SFB_GNB1L	Lenti plasmid for generating GNB1L expressing stable cell lines
224385	pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion	Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines
224387	pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion	Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines
224388	pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion	Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines