CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma.
Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST
Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3. Epub 2023 Jul 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221676 | pcDNA4.1-Myc-RNF8 | Mammalian expression of Myc-tagged human RNF8 |
| 221677 | pcDNA4.1-Myc-RNF8-R42A | Mammalian expression of Myc-tagged human RNF8 (R42A) |
| 221678 | pcDNA4.1-Myc-RNF8-C403S | Mammalian expression of Myc-tagged human RNF8 (C403S) |
| 221679 | pcDNA4.1-Myc-RNF8-S157A | Mammalian expression of Myc-tagged human RNF8 (S157A) |
| 221680 | pcDNA4.1-Myc-RNF8-T198A | Mammalian expression of Myc-tagged human RNF8 (T198A) |
| 221681 | pcDNA4.1-Myc-RNF8-3A | Mammalian expression of Myc-tagged human RNF8 (R477A/E478A/R479A) |
| 221682 | pcDNA4.1-Myc-RNF8-4A | Mammalian expression of Myc-tagged human RNF8 (R477A/E478A/R479A/K480A) |
| 221683 | pcDNA3.1-Myc-BirA*-RNF8 | Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID |
| 221684 | pcDNA3.1-Myc-BirA*-RNF8-R42A | Mammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioID |
| 221685 | pcDNA3.1-Myc-BirA*-RNF8-C403S | Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID |
| 221686 | pcDNA3.1-Myc-BirA*-CAMK2D | Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID |
| 221687 | pCMV-FLAG-CAMK2D | Mammalian expression of FLAG-tagged human CAMK2D |
| 221688 | pCMV-FLAG-CAMK2D-ED | Mammalian expression of FLAG-tagged human CAMK2D (K43R/D136N) |
| 221689 | pCMV-FLAG-CAMK2D-T287A | Mammalian expression of FLAG-tagged human CAMK2D (T287A) |
| 221690 | pCMV-FLAG-MAD2 | Mammalian expression of FLAG-tagged human MAD2 |
| 221691 | pCMV-FLAG-MAD2-RQ | Mammalian expression of FLAG-tagged human MAD2 (R133E/Q134A) |
| 221692 | pCMV-FLAG-p31(comet) | Mammalian expression of FLAG-tagged human p31(comet) |
| 221693 | pCMV-FLAG-p31(comet)-QF | Mammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A) |
| 221694 | pCMV-FLAG-CDC20 | Mammalian expression of FLAG-tagged human CDC20 |
| 221695 | pCMV-FLAG-RNF8 | Mammalian expression of FLAG-tagged human RNF8 |
| 221696 | pCMV4-HA-MAD2 | Mammalian expression of HA-tagged human MAD2 |
| 221697 | pCMV4-HA-p31(comet) | Mammalian expression of HA-tagged human p31(comet) |
| 221698 | pCMV4-HA-p31(comet)-S102A | Mammalian expression of HA-tagged human p31(comet) (S102A) |
| 221699 | plenti-FLAG-CAMK2D | Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells |
| 221700 | plenti-FLAG-CAMK2D-ED | Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells |
| 221702 | pHAGE-RNF8-R42A | Lentiviral expression of human RNF8 (R42A) in mammalian cells |
| 221703 | pHAGE-RNF8-C403S | Lentiviral expression of human RNF8 (C403S) in mammalian cells |
| 221704 | pHAGE-RNF8-S157A | Lentiviral expression of human RNF8 (S157A) in mammalian cells |