TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry.
Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ, Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E, Cherepanov P, Daelemans D
Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237476 | pLCKO-CMV-ACE2-P2A-Blasticidin | Encodes full length human ACE2 protein, along with blasticidin selection marker. |
| 237477 | pLCKO-CMV-TMPRSS2-IRES-Hygromycin | Encodes full length human TMPRSS2 protein, along with hygromycin selection marker. |
| 237478 | pLCKO-CMV-TMEM106B-P2A-Blasticidin | Encodes full length human TMEM106B protein, along with blasticidin selection marker. |
| 237479 | pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin | Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization. |
| 237480 | pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin | Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding. |
| 238130 | pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human | This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker. |
| 238131 | pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster | This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker. |
| 238132 | pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey | This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker. |
| 238133 | pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse | This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker. |