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Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors.

Xiang G, Li Y, Sun J, Huo Y, Cao S, Cao Y, Guo Y, Yang L, Cai Y, Zhang YE, Wang H
Nat Biotechnol. 2023 Jun 29. doi: 10.1038/s41587-023-01857-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
205316pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNAAll-in-one AAV vector expressing ISAam1 TnpB and its reRNA
205317pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNAAll-in-one AAV vector expressing ISYmu1 TnpB and its reRNA
205318pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNAAll-in-one AAV vector expressing ISDge10 TnpB and its reRNA
205319pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNAAll-in-one AAV vector expressing ISTfu1 TnpB and its reRNA
205320pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNAAll-in-one AAV vector expressing ISAba30 TnpB and its reRNA
205321pBad33_IS605_reRNABacterial expression of IS605 TnpB and its reRNA
205322pBad33_ISAam1_reRNABacterial expression of ISAam1 TnpB and its reRNA
205323pBad33_ISAba30_reRNABacterial expression of ISAba30 TnpB and its reRNA
205324pBad33_ISAeme8_reRNABacterial expression of ISAeme8 TnpB and its reRNA
205325pBad33_ISAepa1_reRNABacterial expression of ISAepa1 TnpB and its reRNA
205326pBad33_ISAs26_reRNABacterial expression of ISAs26 TnpB and its reRNA
205327pBad33_ISBce3_reRNABacterial expression of ISBce3 TnpB and its reRNA
205328pBad33_ISBth16_reRNABacterial expression of ISBth16 TnpB and its reRNA
205329pBad33_ISCath1_reRNABacterial expression of ISCath1 TnpB and its reRNA
205330pBad33_ISCbt1_reRNABacterial expression of ISCbt1 TnpB and its reRNA
205331pBad33_ISCbt2_reRNABacterial expression of ISCbt2 TnpB and its reRNA
205332pBad33_ISCco1_reRNABacterial expression of ISCco1 TnpB and its reRNA
205333pBad33_ISClsp11_reRNABacterial expression of ISClsp11 TnpB and its reRNA
205334pBad33_ISClte2_reRNABacterial expression of ISClte2 TnpB and its reRNA
205335pBad33_ISCpe2_reRNABacterial expression of ISCpe2 TnpB and its reRNA
205336pBad33_ISCvi1_reRNABacterial expression of ISCvi1 TnpB and its reRNA
205337pBad33_ISCvi2_reRNABacterial expression of ISCvi2 TnpB and its reRNA
205338pBad33_ISCysp13_reRNABacterial expression of ISCysp13 TnpB and its reRNA
205339pBad33_ISCytsp1_reRNABacterial expression of ISCytsp1 TnpB and its reRNA
205340pBad33_ISDge10_reRNABacterial expression of ISDge10 TnpB and its reRNA
205341pBad33_ISEc41_reRNABacterial expression of ISEc41 TnpB and its reRNA
205342pBad33_ISEc44_reRNABacterial expression of ISEc44 TnpB and its reRNA
205343pBad33_ISEc46_reRNABacterial expression of ISEc46 TnpB and its reRNA
205344pBad33_ISEfa4_reRNABacterial expression of ISEfa4 TnpB and its reRNA
205345pBad33_ISHahl1_reRNABacterial expression of ISHahl1 TnpB and its reRNA
205346pBad33_ISKpn69_reRNABacterial expression of ISKpb69 TnpB and its reRNA
205347pBad33_ISMac7_reRNABacterial expression of ISMac7 TnpB and its reRNA
205348pBad33_ISMma22_reRNABacterial expression of ISMma22 TnpB and its reRNA
205349pBad33_ISMmu1_reRNABacterial expression of ISMmu1 TnpB and its reRNA
205350pBad33_ISNsp2_reRNABacterial expression of ISNsp2 TnpB and its reRNA
205351pBad33_ISNsp3_reRNABacterial expression of ISNsp3 TnpB and its reRNA
205352pBad33_ISRor9_reRNABacterial expression of ISRor9 TnpB and its reRNA
205353pBad33_ISSen6_reRNABacterial expression of ISSen6 TnpB and its reRNA
205355pBad33_ISTfu1_reRNABacterial expression of ISTfu1 TnpB and its reRNA
205356pBad33_ISTva1_reRNABacterial expression of ISTva1 TnpB and its reRNA
205357pBad33_ISVgsp2_reRNABacterial expression of ISVgsp2 TnpB and its reRNA
205358pBad33_ISYmu1_reRNABacterial expression of ISYmu1 TnpB and its reRNA

Antibodies from Article