Skip to main content
Addgene

Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status.

Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. Epub 2023 Jun 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
203479pGWB511-TP-UnaG-FLAGFor Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.
203480pGWB511-TP-BVRAFor Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.
203482pGWB511-TP-tagRFPFor Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.
203483pGWB602-TP-mCherry-UnaGFor Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.
203484pGWT35S-TP-UnaGFor particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.
203485pDEST17-UnaGFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.
203486pDEST17-HO1(51-231)For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.
203760pGWB560-NADK2For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Antibodies from Article