Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag.
Liang SQ, Liu P, Ponnienselvan K, Suresh S, Chen Z, Kramme C, Chatterjee P, Zhu LJ, Sontheimer EJ, Xue W, Wolfe SA
Nat Methods. 2023 Jun;20(6):898-907. doi: 10.1038/s41592-023-01859-2. Epub 2023 May 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201973 | pET-21a-PE2-His | Express His-tagged PE2 in bacteria |
| 201974 | pegRNA_HEK4_20-7 | Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm |
| 201975 | pegRNA_HEK4_G-T | Express a pegRNA used for G to T conversion at HEK4 site |
| 201976 | pegRNA_HEK4_1bp-deletion | Express a pegRNA used for 1bp deletion at HEK4 site |
| 201977 | pegRNA_HEK4_1bp-insertion | Express a pegRNA used for 1bp insertion at HEK4 site |