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An automated proximity proteomics pipeline for subcellular proteome and protein interaction mapping.

Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ, Huttenhain R
bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. Preprint. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215552Cyto-APEX2Stable, inducible expression in cytoplasm
215553Endo-APEX2Stable, inducible expression in endosome
215554GALT-APEX2Stable, inducible expression in Golgi
215555LAMP1-APEX2Stable, inducible expression in lysosome
215556LAMTOR1-APEX2Stable, inducible expression in lysosome
215557PM-APEX2Stable, inducible expression in plasma membrane
2155585HT2A-APEX2Stable, inducible expression of 5HT2A

Antibodies from Article