Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq.
Wessels HH, Mendez-Mancilla A, Hao Y, Papalexi E, Mauck WM 3rd, Lu L, Morris JA, Mimitou EP, Smibert P, Sanjana NE, Satija R
Nat Methods. 2023 Jan;20(1):86-94. doi: 10.1038/s41592-022-01705-x. Epub 2022 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192505 | pLentiRNAGuide_003 | For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP |
| 192506 | lentiGuideFB-Puro-A | For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI. |
| 192507 | lentiGuideFB-Puro-B | For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI. |