The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria.
Gonzalez-Magana A, Altuna J, Queralt-Martin M, Largo E, Velazquez C, Montanchez I, Bernal P, Alcaraz A, Albesa-Jove D
Commun Biol. 2022 Nov 5;5(1):1189. doi: 10.1038/s42003-022-04140-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192948 | pKTop::sptse5-CT (1169-1229) | For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192949 | pKTop::sptse5-CT (1169-1269) | For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192950 | pKTop::sptse5-CT (1169-1281) | For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192951 | pKTop::sptse5-CT (1169-1300) | For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192953 | pKTop::tse5-CT (1169-1229) | For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192954 | pKTop::tse5-CT (1169-1269) | For membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192955 | pKTop::tse5-CT (1169-1281) | For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192956 | pKTop::tse5-CT (1169-1300) | For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192957 | pKTop::tse5-CT (1169-1317) | For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus |
| 192958 | pS238D1::tse5-CT | For expression of Tse5-CT (1169-1317) in P. putida for biological function studies |
| 192959 | pS238D1::sptse5-CT | For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies |
| 192960 | pSEVA424::tsi5 | Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms |
| 192961 | pS238D1::sptse5-CT_phoA-lacZalpha | Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida |
| 192962 | pET29a(+)::9xhis-Tse5 | Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT |
| 192963 | pET29a(+)::D1141A | Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL |