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Stress-induced protein disaggregation in the endoplasmic reticulum catalysed by BiP.

Melo EP, Konno T, Farace I, Awadelkareem MA, Skov LR, Teodoro F, Sancho TP, Paton AW, Paton JC, Fares M, Paulo PMR, Zhang X, Avezov E
Nat Commun. 2022 May 6;13(1):2501. doi: 10.1038/s41467-022-30238-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183686SP_H6_HaloK73T_KDEL_pCMV1Mammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)
183687pCMV_HaloK73T_cytoMammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)
183688SP_H6_Halo_WT_KDEL_pBABEpuRetroviral expression of ER targeted H6-tagged Halotag Wild type
183689SP_H6_Halo_K73T_KDEL_pBABEpuRetroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)
183690SP_H6_Halo_L172Q_KDEL_pBABEpuRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)
183691SP_H6_Halo_M21K_F86L_KDEL_pBABEpuRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)
183692pET30_Halotag_K73T_v2Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)
183693pET30_Halotag_K73T_JdomainBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain
183694pCRISPRa_CHO-Bip_gRNA1CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)
183695pCRISPRa_all-in-oneExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective
183696pCRISPRa_all-in-one_Bip gRNA1Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Antibodies from Article