Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors.
Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, French CE, Wang B
Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184138 | pLY162 | Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1) |
| 184140 | pLY164 | Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3) |
| 184141 | pLY163 | Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2) |
| 184142 | pLY168 | Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2) |
| 184145 | pLY171 | sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa |
| 184146 | pLY185 | RFP generator controlled by Plux2 promoter |
| 184148 | pLY227 | short crRNA generator with spacer LEA2 with WT repeat region |
| 184149 | pLY232 | short tracrRNA generator with WT anti-repeat region |
| 184150 | pLY237 | Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1) |
| 184152 | pLY239 | Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3) |
| 184153 | pLY251 | Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2) |
| 184155 | pLY273 | Reprogrammed short tracrRNA for hijacking mRNA of zraP |
| 184156 | pLY283 | Reprogrammed short tracrRNA for hijacking mRNA of zntA |
| 184158 | pLY300 | Reporter circuit for hijacking small RNA oxyS as CRISPR RNA |
| 184159 | pLY305 | Reporter circuit for hijacking mRNA of zraP as CRISPR RNA |
| 184160 | pLY315 | Reporter circuit for hijacking mRNA of zntA as CRISPR RNA |
| 184161 | pLY320 | Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA |