DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome wide.
Altemose N, Maslan A, Smith OK, Sundararajan K, Brown RR, Mishra R, Detweiler AM, Neff N, Miga KH, Straight AF, Streets A
Nat Methods. 2022 Jun;19(6):711-723. doi: 10.1038/s41592-022-01475-6. Epub 2022 Apr 8.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174372 | pET-pA-Hia5 | SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq) |
| 174373 | pET-pAG-Hia5 | Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq) |
| 222303 | prhaBAD-pA-Hia5 | Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq) |