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Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host.

D'Angelo F, Fernandez-Fueyo E, Garcia PS, Shomar H, Pelosse M, Manuel RR, Buke F, Liu S, van den Broek N, Duraffourg N, de Ram C, Pabst M, Bouveret E, Gribaldo S, Py B, Ollagnier de Choudens S, Barras F, Bokinsky G
Elife. 2022 Mar 4;11:e70936. doi: 10.7554/eLife.70936. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
195055pBbA5a-sufCDSUBExpresses SUF operon from B. subtilis from IPTG-inducible promoter
195056pBbA5a-sufABCDSEExpresses SUF operon from E. coli from IPTG-inducible promoter

Antibodies from Article