Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons.
Wimmer F, Mougiakos I, Englert F, Beisel CL
Mol Cell. 2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 10.1016/j.molcel.2022.01.026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178735 | pAc2_Cas8 | Expression of A. chroococcum type I-E #2 cas8 |
| 178736 | pAc2_Cse2 | Expression of A. chroococcum type I-E #2 cse2 |
| 178737 | pAc2_Cas5 | Expression of A. chroococcum type I-E #2 cas5 |
| 178738 | pAc2_Cas6 | Expression of A. chroococcum type I-E #2 cas6 |
| 178739 | pAc2_Cas7 | Expression of A. chroococcum type I-E #2 cas7 |
| 178740 | pAc2_gRNA1 | Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system |
| 178741 | pAc3_Cas8 | Expression of A. chroococcum type I-E #3 cas8 |
| 178742 | pAc3_Cse2 | Expression of A. chroococcum type I-E #3 cse2 |
| 178743 | pAc3_Cas5 | Expression of A. chroococcum type I-E #3 cas5 |
| 178744 | pAc3_Cas6 | Expression of A. chroococcum type I-E #3 cas6 |
| 178745 | pAc3_Cas7 | Expression of A. chroococcum type I-E #3 cas7 |
| 178746 | pAc3_gRNA1 | Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system |
| 178747 | pLm_Cas8 | Expression of L. mobilis type I-E cas8 |
| 178748 | pLm_Cse2 | Expression of L. mobilis type I-E cse2 |
| 178749 | pLm_Cas5 | Expression of L. mobilis type I-E cas5 |
| 178750 | pLm_Cas6 | Expression of L. mobilis type I-E cas6 |
| 178751 | pLm_Cas7 | Expression of L. mobilis type I-E cas7 |
| 178752 | pLm_gRNA1 | Expression of a gRNA that targets next to PAM library, used for L. mobilis type I-E system |
| 178753 | pEh_Cas8 | Expression of E. haloalkaliphila type I-E cas8 |
| 178754 | pEh_Cse2 | Expression of E. haloalkaliphila type I-E cse2 |
| 178755 | pEh_Cas5 | Expression of E. haloalkaliphila type I-E cas5 |
| 178756 | pEh_Cas6 | Expression of E. haloalkaliphila type I-E cas6 |
| 178757 | pEh_Cas7 | Expression of E. haloalkaliphila type I-E cas7 |
| 178758 | pEh_gRNA1 | Expression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system |
| 178759 | pMs_Cas8 | Expression of Marinomonas sp. type I-E cas8 |
| 178760 | pMs_Cse2 | Expression of Marinomonas sp. type I-E cse2 |
| 178761 | pMs_Cas5 | Expression of Marinomonas sp. type I-E cas5 |
| 178762 | pMs_Cas6 | Expression of Marinomonas sp. type I-E cas6 |
| 178763 | pMs_Cas7 | Expression of Marinomonas sp. type I-E cas7 |
| 178764 | pMs_gRNA1 | Expression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system |
| 178765 | pXalb_IC_Cascade | Expression of X. albilineans type I-C Cascade genes |
| 178766 | pXalb_IC_Cas3 | Expression of X. albilineans type I-C cas3 |
| 178767 | Xalb_I-C_array_GG | Golden Gate vector to generate X. albilineans type I-C single arrays |
| 178768 | pXalb_IF1_Cascade | Expression of X. albilineansCascade type I-F1 Cascade genes |
| 178769 | pXalb_IF1_Cas2-3 | Expression of X. albilineans type I-F1 cas2-3 |
| 178770 | Xalb_I-F1_array_GG | Golden Gate vector to generate X. albilineans type I-F1 single arrays |
| 178771 | pRoCascade_gfp | Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis |
| 178772 | pRoTnsABCDQ | Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis |
| 178773 | pRoDonor | Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis |
| 178774 | pRoTarget | partial tRNA-Leugene from Rippkaea orientalis |
| 178775 | Sth_I-E array_GFP dropout | Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays |
| 178776 | pSth_Cas8 | Expression of S. thermophilus type I-E cas8 |
| 178777 | pSth_Cse2 | Expression of S. thermophilus type I-E cse2 |
| 178778 | pSth_Cas5 | Expression of S. thermophilus type I-E cas5 |
| 178779 | pSth_Cas6 | Expression of S. thermophilus type I-E cas6 |
| 178780 | pSth_Cas7 | Expression of S. thermophilus type I-E cas7 |