High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.
Sridharan S, Gajowa MA, Ogando MB, Jagadisan UK, Abdeladim L, Sadahiro M, Bounds HA, Hendricks WD, Turney TS, Tayler I, Gopakumar K, Oldenburg IA, Brohawn SG, Adesnik H
Neuron. 2022 Jan 27. pii: S0896-6273(22)00008-3. doi: 10.1016/j.neuron.2022.01.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170160 | pCAG-ChroME2f-mRuby2-ST | Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 170161 | pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3 | Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 170163 | pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 | Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 170175 | pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3 | Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 170177 | pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3 | Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 171150 | pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3 | Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 171154 | pCAG-mRuby3 | Encodes mRuby3 fluorophore |
| 171156 | pCAG-GCaMP6s | Encodes GCaMP6s |
| 171158 | pCAG-ChroMD-mRuby2-ST | Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |