Comprehensive interrogation of the ADAR2 deaminase domain for engineering enhanced RNA editing activity and specificity.
Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170124 | MCP-ADAR2-DD | Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal |
| 170125 | MCP-ADAR2-DD(E488Q) | Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal |
| 170126 | MS2-adRNA (5'UAG) | Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125 |
| 170127 | MS2-adRNA (5'GAC) | Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125 |
| 170128 | MS2-adRNA (3'UAG) | Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125 |
| 170129 | MS2-adRNA (3'GAC) | Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125 |
| 170136 | All-in-one split-ADAR2 | AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal |
| 170137 | All-in-one split ADAR2(E488Q) | AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal |
| 170138 | BoxB-MS2 adRNA (RAB7A-UAG site) | AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript |
| 170139 | MCP-ADAR2-DD(E488Q, N496F) | AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal |
| 170140 | MS2-adRNA (RAB7A-GAC site) | AAV vector carrying a MS2 adRNA targeting the RAB7A transcript |
| 170148 | All-in-one split-ADAR2(E488Q, N496F) | AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal |
| 170149 | BoxB-MS2 adRNA (RAB7A-GAC site) | AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript |
| 170153 | Split-RESCUE-N | AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal |
| 170154 | Split-RESCUE-C | AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal |
| 170155 | BoxB-MS2 adRNA (RAB7A-ACG site) | AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing |