Mapping the genetic landscape of DNA double-strand break repair.
Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, Simpson D, Yang D, Bothmer A, Cotta-Ramusino C, Weissman JS, Adamson B
Cell. 2021 Oct 28;184(22):5653-5669.e25. doi: 10.1016/j.cell.2021.10.002. Epub 2021 Oct 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173042 | pAX198 | Custom vector for sgRNA library construction |
| 177168 | pJL056 | Expresses wildtype MRE11 in human cells |
| 177169 | pJL057 | Expresses a nuclease dead MRE11 in human cells |
| 177210 | pJL059 | Bicistronic vector with GFP reporter |
| 177663 | DNA repair library with 1,573 sgRNAs | CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes |
| 177664 | DNA repair library with 336 sgRNAs | CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes |