The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression.
Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA
Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175412 | pCDNA4TO-3XFLAG Halo WT nsp1 | Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175417 | pCDNA4TO-3XFLAG Halo R124A/K125A nsp1 | Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175421 | pCDNA4TO-3XFLAG Halo K164A/H165A nsp1 | Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175422 | pCDNA4TO-3XFLAG Halo R99A nsp1 | Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175423 | pCDNA4TO-3XFLAG Halo delta 1-117 nsp1 | Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175424 | pCDNA4 3xFLAG Halo delta 118-180 nsp1 | Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175425 | pCDNA4 3xFLAG Halo delta 122-130 nsp1 | Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175426 | pCDNA4 3xFLAG Halo G-Linker nsp1 | Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175427 | pCDNA4 3xFLAG Halo E55A E57A K58A nsp1 | Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175428 | pCDNA4 3xFLAG Halo R119A K120A nsp1 | Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175429 | pCDNA4 3xFLAG Halo E36A E37A nsp1 | Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1. |
| 175430 | pJP-CoV2leader-nLuc-TSS | Expresses nLuc with 5' leader of CoV2 in mammalian cells. |
| 175431 | pJP-HBB-nLuc | Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells. |
| 175432 | pUC57-HBB-nLuc | Used to generate IVT RNA for translation extracts |
| 175512 | pGEX nsp1 CoV2 | For protein expression of WT nsp1 CoV2 |
| 175513 | pGEX nsp1 R124A/K125A CoV2 | For protein expression of R124A/K125A nsp1 CoV2 |
| 175514 | pGEX nsp1 K164A/H165A CoV2 | For protein expression of K164A/H165A nsp1 CoV2 |
| 175515 | pGEX nsp1 R99A CoV2 | For protein expression of R99A nsp1 CoV2 |
| 175516 | pGEX C_K nsp1 CoV2 | Expresses C_K for bioconjugation on nsp1 |
| 175517 | pGEX C_K nsp1 K164A/H165A CoV2 | Expresses C_K for bioconjugation on nsp1 |
| 175518 | pUC Ad VAI | For mammalian expression of adenovirus VAI RNA |
| 176057 | pCDNA4 CoV-2 nsp1 3xFLAG | Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus. |
| 176059 | pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG | Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1. |
| 176060 | pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG | Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1. |
| 176061 | pCDNA4TO R99A CoV-2 nsp1 3xFLAG | Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1. |