The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases.

Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F
Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176534	pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep	Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
176535	pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet	Non-protein coding locus components from CRISPR-associated IscB
176536	pHS0663 pET45b(+) KraIscB-1 TwinStrep	Bacterial expression of KraIscB-1-TwinStrep
176537	pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1	Non-protein coding locus components from KraIscB-1
176538	pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA	Mammalian expression of nuclear localized AwaIscB
176539	pHS0806 His14-bdSUMO-AwaIscB	Bacterial expression of His14-bdSUMO-AwaIscB
176540	pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA	Mammalian expression of nuclear localized OgeuIscB
176541	pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold	human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning
176542	pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension	Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus
176587	pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB	Bacterial expression of His14-MBP-TEV-AmaTnpB
176588	pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer	Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer
176589	pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer	Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer