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The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis.

Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH
Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
169296SIN40C.EFS.eGFP.ARID3A-3'UTR-MutConstitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
169297SIN40C.EFS.eGFP.ARID3A-3'UTR-WT Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
169298SIN40C.EFS.eGFP.mArid3a-3'UTR-MutConstitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
169299SIN40C.EFS.eGFP.mArid3a-3'UTR-WT Constitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
169300SIN40C.SFFV.ARID3a.IRES.GFPConstitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein
169301SIN40C.SFFV.dTomato.let-7cConstitutive overexpression of let-7c with dTomato as a reporter fluorescent protein
169302SIN40C.SFFV.dTomato.miR-125b-2Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein
169303SIN40C.SFFV.dTomato.miR-ctrlConstitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein
169304SIN40C.SFFV.GFP.miR-99aConstitutive overexpression of miR-99a with GFP as a reporter fluorescent protein
169305SIN40C.SFFV.GFP.miR-ctrlConstitutive overexpression of miR-ctrl with GFP as a reporter fluorescent protein
169306SIN40C.SFFV.KRAB-mArid3a.IRES.GFPConstitutive overexpression of murine KRAB-Arid3a fusion protein with GFP as reporter fluorescent protein
169307SIN40C.SFFV.Luciferase.IRES.dTomatoConstitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein
169308SIN40C.SFFV.Luciferase.IRES.GFPConstitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein
169309SIN40C.SFFV.mArid3a.IRES.GFPConstitutive overexpression of murine Arid3a cDNA with GFP as reporter fluorescent protein
169310SIN40C.SFFV.mTagBFP2.let-7cConstitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein
169311SIN40C.SFFV.mTagBFP2.miR-125b-2Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein
169312SIN40C.SFFV.mTagBFP2.miR-ctrlConstitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein
169313SIN40C.SFFV.VP64-mArid3a.IRES.GFPConstitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent protein
169314SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of human ARID3A cDNA with dTomato as reporter fluorescent protein
169315SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent protein
169316SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein
169317SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein
169318SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein
174666SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3GDoxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

Antibodies from Article