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Knock-in tagging in zebrafish facilitated by insertion into non-coding regions.

Levic DS, Yamaguchi N, Wang S, Knaut H, Bagnat M
Development. 2021 Sep 8. pii: 272090. doi: 10.1242/dev.199994. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174021pUC19-TgKI-MCS-mLanYFP-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174022pUC19-TgKI-MCS-tdTomato-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174023pUC19-TgKI-MCS-mScarlet-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174024pUC19-TgKI-MCS-eGFP-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174025pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174026pUC19-TgKI-MCS-p2A-QF2-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174027pUC19-TgKI-MCS-p2A-tdTomato-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174028pUC19-TgKI-MCS-p2A-eGFP-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174029pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.
174030pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyAThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Antibodies from Article