Programmable RNA targeting with the single-protein CRISPR effector Cas7-11.

Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS
Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
172500	pDF0039 DisCas7-11 only expression with CRISPR array	Encodes the DisCas7-11 protein only for bacterial expression
172501	pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer	Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers
172502	pDF0044 CjcCas7-11 full locus with synthetic array	Encodes the CjcCas7-11 full locus for bacterial expression
172503	pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon	Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification
172504	pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon	Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification
172505	pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon	Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification
172506	pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides	Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
172507	pDF0159 pCMV - huDisCas7-11 mammalian expression	Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells
172508	pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site	Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning
172509	pDF0234 pCMV - huDisCas7-11 dead mutant	Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells
172510	pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2	Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells
172511	pDF0317 HvsCas7-11 mammalian expression	Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells
172514	pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector	Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter
172515	pDF0336 pAAV EFS-HvsCas7-11 for dual vector	Encodes HvsCas7-11 mammalian expression casette in AAV backbone
172516	pDF0352 HvsCas7-11 with C-term Nes mammalian expression	Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells