Skip to main content
Addgene

Efficient precise in vivo base editing in adult dystrophic mice.

Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han R
Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
187053pLKO‐puro‐2A‐mdx4cv‐GFPfluorescent reporter for adenine base editing of mdx4cv point mutation
187054pLenti‐puro‐OgRNA_mdxE53SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
187055pCMV_ABEmaxNG‐NGAABEmax-SpCas9-NGA
187056pCMV_ABE‐NGCABEmax-SpCas9-NGC
187057pCMV_ABE‐NG‐loopABEmax-SpCas9-NG-loop
187058pCMV‐ABEmaxNGXABEmax-SpCas9-NGX
187059pCMV‐ABEmaxNGX‐NGAABEmax-SpCas9-NGX‐NGA
187060pCMV‐ABEmaxNGX‐NGCABEmax-SpCas9-NGX-NGC
187061pCMV‐ABEmaxNGX‐loopABEmax-SpCas9-NGX-loop
187062pCMV_miniABEmaxNG(GG)miniABEmax(GG)-SpCas9-NG
187063pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNANpu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187064pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNANpu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187065pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNAGp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187066pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNAGp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187067pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation
187068pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNAGp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation
187069pCMV_SpG‐ABE8e(V106W)ABE8e(V106W)-SpG

Antibodies from Article