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Organelle degradation in the lens by PLAAT phospholipases.

Morishita H, Eguchi T, Tsukamoto S, Sakamaki Y, Takahashi S, Saito C, Koyama-Honda I, Mizushima N
Nature. 2021 Apr;592(7855):634-638. doi: 10.1038/s41586-021-03439-w. Epub 2021 Apr 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167987pMRX-IP-EGFP-mouse Plaat3 (WT)Expresses EGFP-Plaat3 in mammalian cells
168771pMRX-IP-EGFP-mouse Plaat3 (N157stop)Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells
168772Tol2-aACry::EGFP-zebrafish Plaat1 (WT)Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens
169000pCS2-ER-EmEGFPExpress ER-EmEGFP in zebrafish
169001pCS2-Mito-mRFPExpress Mito (Su9)-mRFP in zebrafish
169002pCS2-Dnase1l1l-mRFPExpress zebrafish Dnase1l1l-mRFP in zebrafish

Antibodies from Article