Recording of elapsed time and temporal information about biological events using Cas9.
Park J, Lim JM, Jung I, Heo SJ, Park J, Chang Y, Kim HK, Jung D, Yu JH, Min S, Yoon S, Cho SR, Park T, Kim HH
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164124 | Lenti_stgRNA-Puro | Backbone for lentiviral stgRNA library construction (library 1 and 2) |
| 164131 | pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP | Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines) |
| 164132 | pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP | Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines) |
| 164133 | Lenti_CMV-Cas9-P2A-HygR | Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells |
| 164134 | Lenti_EFS-Cas9-P2A-HygR | Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells |
| 164135 | Lenti_PGK-Cas9-P2A-HygR | Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells |
| 164136 | Lenti-pHSE::Cre-mRuby3 | Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction |
| 164137 | Lenti-pNFkB::Cre-mRuby3 | Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa |
| 164138 | Cre-responsive Cas9 expressing transposon library vector | Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.) |