Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
Heinz MC, Oost KC, Snippert HJG
STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136257 | pLV 4xSTAR-mTFP-NLS-blast | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136258 | pLV 4xSTAR-mNeonGreen-NLS-blast | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136259 | pLV 4xSTAR-sTomato-NLS-blast | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136260 | pLV 4xSTAR-mScarletI-NLS-blast | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136261 | Tol2 8xSTAR-sTomato-NLS. PGK-puro | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136262 | Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136263 | Tol2 8xSTAR-mScarletI-NLS. PGK-puro | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136264 | Tol2 8xSTAR-sTomato. PGK-puro | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 136266 | Tol2 8xSTAR-mScarletI. PGK-puro | Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity |
| 158774 | CMV-Tol2 transposase | Expression of Tol2 transposase |