High-Throughput Discovery and Characterization of Human Transcriptional Effectors.
Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik MC, Bintu L
Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161925 | pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE | Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR |
| 161926 | pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE | Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG |
| 161927 | pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus |
| 161928 | pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus |
| 161929 | pJT040 lenti MCS pEF mIFP WPRE | Lentiviral expression of mIFP with multiple cloning site upstream the pEF promoter |