Escherichia coli amino acid auxotrophic expression host strains for investigating protein structure-function relationships.

Iwasaki T, Miyajima-Nakano Y, Fukazawa R, Lin MT, Matsushita SI, Hagiuda E, Taguchi AT, Dikanov SA, Oishi Y, Gennis RB
J Biochem. 2021 Apr 29;169(4):387-394. doi: 10.1093/jb/mvaa140. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
102799	RF15	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.
102800	RF16	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.
102801	RF17	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.
102802	RF18	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.
102803	RF21	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)
102804	EH1	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.