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Opposing Effects of Cohesin and Transcription on CTCF Organization Revealed by Super-resolution Imaging.

Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
161972PB-Ostir-puroExpresses Ostir and puromycin resistance gene in mammalian cells
161973PB-Ostir-neoExpresses Ostir and G418 resistance gene in mammalian cells
161974PX458-mcherryExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA
161975pGEMT-GFP-p2a-aidhomologous donor template for endogenous tagging
161976pGEMT-aid-p2a-GFPhomologous donor template for endogenous tagging
161977pGEMT-halo-HA-p2a-neoRhomologous donor template for endogenous tagging
161979pGEMT-puroR-p2a-HA-halohomologous donor template for endogenous tagging
161980pGEMT-blastR-p2a-v5-dL5homologous donor template for endogenous tagging
161981pGEMT-v5-dL5-p2a-blastRhomologous donor template for endogenous tagging

Antibodies from Article