The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.
Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orlowski A, Janis J, Maatta JAE, Varjosalo M, Vattulainen I, Rog T, Svergun D, Cheng RH, Wu J, Hytonen VP, Wehrle-Haller B
J Cell Sci. 2020 Oct 12;133(19). pii: 133/19/jcs239202. doi: 10.1242/jcs.239202.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166127 | t1-405 | For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal. |
| 166128 | t1-405(del30) | Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal. |
| 166129 | t1-405(del37/GAG) | Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site. |
| 166130 | t1-405(T144E,T150E) | For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal. |
| 166131 | t1-405(151-154AAAA) | Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site. |
| 166132 | t206-405 | For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal. |