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The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.

Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales E, Glaunsinger BA
PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 10.1371/journal.ppat.1008843. eCollection 2020 Sep. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138420pcDNA4/TO-ORF24 1-201-3xFLAGExpresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells
138421pcDNA4/TO-ORF24 1-271-3xFLAGExpresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells
138422pcDNA4/TO-ORF24 1-133-3xFLAGExpresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells
138423pcDNA4/TO-ORF24-3xFLAGExpresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells
138424pcDNA4/TO-ORF24 202-752-3xFLAGExpresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells
138425pcDNA4/TO-ORF24 3L_A-3xFLAGExpresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cells
138426pcDNA4/TO-ORF24 L73A-3xFLAGExpresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cells
138427pcDNA4/TO-ORF24 L74A-3xFLAGExpresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cells
138428pcDNA4/TO-ORF24 L75A-3xFLAGExpresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cells
138429pcDNA4/TO-ORF24 L73A_L75A-3xFLAGExpresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells
138430pcDNA4/TO-ORF24 L74A_L75A-3xFLAGExpresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells
138431pcDNA4/TO-ORF24 R68A-3xFLAGExpresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cells
138432pcDNA4/TO-ORF24 R72A-3xFLAGExpresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cells
138433pcDNA4/TO-ORF24 L73A_L74A-3xFLAGExpresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cells
138434pcDNA4/TO-UL87-2xStrepExpresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells
138435pcDNA4/TO-mu24-2xStrepExpresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cells
138436pcDNA4/TO-BcRF1-2xStrepExpresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells
138437pcDNA4/TO-UL87 3L_A-2xStrepExpresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cells
138438pcDNA4/TO-mu24 3L_A-2xStrepExpresses full-length mu24 3L_A with a C-terminal 2xStrep tag in mammalian cells
138439pcDNA4/TO-BcRF1 3L_A-2xStrepExpresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cells
138440pcDNA4/TO-ORF24 3L_A-2xStrepExpresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cells
138441pcDNA4/TO-ORF24 1-191-2xStrepExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells
138442pcDNA4/TO-ORF24 1-201-2xStrepExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells
138443pcDNA4/TO-ORF24 1-226-2xStrepExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells
138444pcDNA4/TO-mu24 1-181-2xStrepExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells
138445pcDNA4/TO-mu24 1-191-2xStrepExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells
138446pcDNA4/TO-mu24 1-216-2xStrepExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells
138447pcDNA4/TO-BcRF1 1-168-2xStrepExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells
138448pcDNA4/TO-BcRF1 1-178-2xStrepExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells
138449pcDNA4/TO-BcRF1 1-203-2xStrepExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells
138450pcDNA4/TO-UL87 1-238-2xStrepExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells
138451pcDNA4/TO-UL87 1-248-2xStrepExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells
138452pcDNA4/TO-UL87 1-273-2xStrepExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells
138453pcDNA4/TO-mu24-ORF24-2xStrepExpresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells
138454pcDNA4/TO-BcRF1-ORF24-2xStrepExpresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells
138455pcDNA4/TO-UL87-ORF24-2xStrepExpresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells
138464pGEX4T1-ORF24-NTDExpresses GST-tagged ORF24-NTD in bacteria
138465pMAL-c2X-ORF24-NTDExpresses MBP-tagged ORF24-NTD in bacteria
138466pMAL-c2X-ORF24-NTD-3L_AExpresses MBP-tagged ORF24-NTD 3L_A in bacteria
138467p6H-SUMO3-ORF24-NTD-StrepExpresses SUMO3-tagged ORF24-NTD-Strep in bacteria
138468pGEX4T1-Rpb1-linkerExpresses GST-tagged Rpb1 linker in bacteria
138470pQLink-GST-2xCTDExpresses GST-tagged 2xCTD heptapeptide repeat in bacteria
138471pQLink-GST-4xCTDExpresses GST-tagged 4xCTD heptapeptide repeat in bacteria
138472pQLink-GST-10xCTDExpresses GST-tagged 10xCTD heptapeptide repeat in bacteria
138484pGEX4T1-Rpb4/7Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

Antibodies from Article