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Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells.

Moudgil A, Wilkinson MN, Chen X, He J, Cammack AJ, Vasek MJ, Lagunas T Jr, Qi Z, Lalli MA, Guo C, Morris SA, Dougherty JD, Mitra RD
Cell. 2020 Aug 20;182(4):992-1008.e21. doi: 10.1016/j.cell.2020.06.037. Epub 2020 Jul 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154884PB-SRT-PuropiggyBac self-reporting transposon with puromycin marker for mammalian calling cards
154885PB-SRT-tdTomatopiggyBac self-reporting transposon with tdTomato marker for mammalian calling cards
154887SP1-SB100XSleeping Beauty transposase fused to SP1
154888SB-SRT-PuroSleeping Beauty self-reporting transposon with puromycin reporter
154890BRD4 CRISPRi plasmidHuman BRD4 CRISPRi gRNA

Antibodies from Article