5' modifications to CRISPR-Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage.

Mullally G, van Aelst K, Naqvi MM, Diffin FM, Karvelis T, Gasiunas G, Siksnys V, Szczelkun MD
Nucleic Acids Res. 2020 Jul 9;48(12):6811-6823. doi: 10.1093/nar/gkaa477. PubMed Article

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