Computational design of anti-CRISPR proteins with improved inhibition potency.
Mathony J, Harteveld Z, Schmelas C, Upmeier Zu Belzen J, Aschenbrenner S, Sun W, Hoffmann MD, Stengl C, Scheck A, Georgeon S, Rosset S, Wang Y, Grimm D, Eils R, Correia BE, Niopek D
Nat Chem Biol. 2020 Apr 13. pii: 10.1038/s41589-020-0518-9. doi: 10.1038/s41589-020-0518-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128112 | AcrIIC1X | AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9 |
| 128113 | AcrIIC1-mCherry chimera #10 | Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9 |
| 128114 | AcrIIC1X* (AcrIIC1X-mCherry chimera) | Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9 |