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Engineered symbionts activate honey bee immunity and limit pathogens.
Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, Davies BW, Ellington AD, Barrick JE, Moran NA
Science. 2020 Jan 31;367(6477):573-576. doi: 10.1126/science.aax9039. Epub 2020 Jan 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183126pBTK150BTK Type 2 promoter part for golden gate assembly
183127pBTK151BTK Type 4 reverse promoter part for golden gate assembly
183129pBTK562 (pDS-GFP)Control plasmid that expresses off-target dsRNA
183130pBTK1023 (pDS-InR1)Expresses dsRNA targeting the InR1 gene from Apis mellifera
183131pBTK1017 (pNR)Control plasmid that does not express dsRNA

Antibodies from Article