IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints.
Panda SK, Wigerblad G, Jiang L, Jimenez-Andrade Y, Iyer VS, Shen Y, Boddul SV, Guerreiro-Cacais AO, Raposo B, Kasza Z, Wermeling F
Proc Natl Acad Sci U S A. 2020 Jan 24. pii: 1914186117. doi: 10.1073/pnas.1914186117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137729 | LentiGuide Puro-P2A-EGFP | For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection). |
| 137730 | LentiGuide-Puro-P2A-EGFP_mRFPstuf | Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA |