An engineered enzyme that targets circulating lactate to alleviate intracellular NADH:NAD(+) imbalance.
Patgiri A, Skinner OS, Miyazaki Y, Schleifer G, Marutani E, Shah H, Sharma R, Goodman RP, To TL, Robert Bao X, Ichinose F, Zapol WM, Mootha VK
Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0377-7. doi: 10.1038/s41587-019-0377-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139971 | pET30a-His6-ABP-LOXCAT | Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP). |
| 139972 | pET30a-His6-ABP-LOXCATmut | A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A) |
| 140084 | pET30a-His6-LOXCAT | Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. |
| 140085 | pET30a-His6-LOXCATmut | A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A) |