A new vector system for targeted integration and overexpression of genes in the crop pathogen Fusarium solani.
Nielsen MR, Holzwarth AKR, Brew E, Chrapkova N, Kaniki SEK, Kastaniegaard K, Sorensen T, Westphal KR, Wimmer R, Sondergaard TE, Sorensen JL
Fungal Biol Biotechnol. 2019 Dec 11;6:25. doi: 10.1186/s40694-019-0089-2. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133094 | pSHUT4-eYFP | Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI |